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E-learning or electronic learning can be defined as learning through the use of technology such as the Internet instead of teaching in a traditional classroom setting. This form of learning is usually associated with educational institutions as traditionally it is seen as a complement to brick-and-mortar environment. In recent years, many organisations have included e-learning as part of the training regime of its employees. Many reasons form the basis for this inclusion especially the relatively lower cost of training and the mobility of such trainings. In this research study, researcher tried to focus on the implementation of E-learning to employees’ performance in organisations. The purpose of this study is to identify the factors that influence the effectiveness of E-learning within organisations. The five independent variables identified are employees’ attitude, perceived ease of use, instructors’ quality, technology quality and E-learning course content all of which can be a measure for the effectiveness of E-learning in employees’ performance. A survey was conducted among open distance learning students of the Kuala Lumpur Regional Centre who have prior experience in e-learning for training. The results show that instructor’s quality and the e-learning content are the most significant contributors to the effectiveness of the e-learning environment for employee training. The results from this study can be as a reference to future researchers who like to emphasize on the implementation of E-learning towards the employees’ performance.